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KMID : 0389420090170030313
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Korean Journal of Stress Research
2009 Volume.17 No. 3 p.313 ~ p.321
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Effect of N-acetylcysteine on Hydrogen Peroxide-Induced Apoptosis in Mouse C2C12 Myoblast Cells
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Lee Choong-Yeol
Jang Mi-Hyeon
Kim Ye-Jin
Kim Chang-Ju
Shin Mal-Soon
Koh Ji-Eun
Kim Tae-Woon
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Abstract
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Reactive oxygen species (ROS) plays an important role as a mediator of skeletal muscle damage after strenuous exercise and during aging. In the present study, the protective effect of the antioxidant N-acetylcysteine (NAC) against hydrogen peroxide (H2O2)-induced cytotoxicity in myoblast C2C12 cells was investigated. Through morphological and biochemical analysis, it was demonstrated that C2C12 cells treated with H2O2 exhibited several features of apoptosis. The apoptotic characteristic was decreased in cells treated with NAC prior to H2O2 exposure. In addition, it was shown that H2O2 increased the expression of bax but decreased the expression of bcl-2. Pre-treatment with NAC intervened H2O2-induced increase in the bax expression level and alleviated H2O2-induced suppression of bcl-2 expression. Our data show that NAC protects myoblast C2C12 cells against H2O2-induced apoptosis through the regulation of the expression of bax and bcl-2.
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KEYWORD
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N-acetylcysteine, Reactive oxygen species, Hydrogen peroxide, Skeletal muscle cells, Apoptosis
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